CONCEPTS AND PROCEDURES OFCONTEMPORARY CHEMICAL ANALYSISIII. EVALUATION OF MOLECULAR UV - VISSPECTROPHOTOMETRY.

Abstract

In the present article a critical discussion is made about the UV – Visible spectrophotometric methods of chemical analysis based on its physicochemical principles, potentialities and analytical characteristics, as well as some of its representative applications in industry and environmental control. These analysis methods were developed due to the need to increase analytical characteristics such as sensitivity, selectivity, limit of detection and speed in the analysis in order to be applied in the trace analysis, where the classical methods no longer have sensitivity. Despite its worst accuracy (1 - 5% DER), detection limits of less than 10-5 M are common with analytical capacities of dozens of samples per hour. The analytical speed can be seen re-powered up to a factor of 10 with FIA couplings, which are necessary in routine analyzes of a large number of samples. The great versatility of the spectrophotometric methodology allows, then, the determination of a great variety of analytes in diversity of matrices with great accuracy and speed. It is concluded that it is necessary to disseminate and teach students and professionals related to areas of knowledge dependent on chemical analysis.